Celebrity Autobiography presents a rotating cast of performers reading aloud from the published memoirs of other well-known figures, staging the material as live comedy. Solo excerpts alternate with ensemble pieces that combine passages from multiple autobiographies, producing scenes built around conflicting accounts, famous relationships, and divergent recollections of shared experiences. Each performance runs approximately 90 minutes, with the cast and material shifting throughout the run.

The Broadway production draws on a lineup of over 40 performers appearing across the engagement, including Christie Brinkley, Mario Cantone, Chloe Fineman, Kathy Griffin, Jeff Hiller, Jackie Hoffman, Ken Jeong, Gayle King, Susan Lucci, Ralph Macchio, Andrea Martin, Laraine Newman, Billy Porter, Tony Shalhoub, Sherri Shepherd, Molly Shannon, Kenan Thompson, Jennifer Tilly, Nia Vardalos, and Rita Wilson, among others. Individual performers appear for varying durations, with additional guest appearances scheduled throughout the run.